Improved recovery of a radiolabeled peptide with an albumin-treated reversed-phase HPLC column.

improved Recovery of a Radlolabeled Peptide with an Albumin - Treated Reversed - Phase HPLC

The results in Figure la were obtained with a column that had already been used in >300 protein and peptide purifications. When we replaced this with a new column from the same manufacturer, there was a dramatic decrease in recovery of the labeled peptide (Figure lb), as shown by the absence of the 1-34 PTHrP peak at 29 mm. Such behavior might be caused by strong or irreversible binding of pept...

Improved Recovery of a Radlolabeled Peptide with anAlbumin-Treated Reversed-Phase HPLC Column

IMPROVED CHROMATOGRAPHIC METHOD FOR DETERMINATION OF SODIUM DICLOFENAC IN INJECTABLE SOLUTION AND PREDICTION OF CHEMICAL STABILITY

Two modified methods for assaying sodium diclofenac were developed by GC and HPLC. Diclofenac was converted into methyl ester derivative by methyl iodide in acetone. The ester was extract and subjected to GLC with flame ionization detector. %5 SE-30/chrom W-HP (80-100 mesh) was used as a column in GC. For reversed phase HPLC, the mobile phase was methanol and water (55-45). The separation was p...

A Reversed Phase High Performance Liquid Chromatographic Method for Determination of Rapamycin

Easily degradating and various isomeric forms of rapamycin (Sirolimus) face the determination of this compound to many challenges. In this study, we developed and validated the isocratic reversed phase high performance liquid chromatographic (RP-HPLC) method for rapamycin. Separation was performed on a C8 column (MZ, 15 × 4.6 mm, 5 µm particle size) using methanol:water (80:20 v/v) as the mobil...

A Reversed Phase High Performance Liquid Chromatographic Method for Determination of Rapamycin

Easily degradating and various isomeric forms of rapamycin (Sirolimus) face the determination of this compound to many challenges. In this study, we developed and validated the isocratic reversed phase high performance liquid chromatographic (RP-HPLC) method for rapamycin. Separation was performed on a C8 column (MZ, 15 × 4.6 mm, 5 µm particle size) using methanol:water (80:20 v/v) as the mobil...